AFM of marked DNA molecules. (A) A diagrammatic representation of the DNA molecules (RsaI-digested pSP64 DNA fragment) that were marked for AFM observation. (B) A typical AFM pattern of the marked DNA molecules. Arrowheads indicate the position of the marked (multistranded) sequence in the DNA molecules. Scale markers indicate 200 nm (a,b,c) and 100 nm (d), respectively. (C) The distribution pattern of the marked sequence as a function of its relative distance from the nearest end of the DNA molecules. The broken line represents the position of the marked region estimated from the location of the sequence in the DNA molecules (see A). A total of 150 molecules were subjected to the analysis. For details, see Methods.
