Direct marking with different probing oligonucleotides. DNA fragments with various sizes (300, 500, 900, and 1200 bp) of pSP64 DNA were subjected to direct marking using four different probing oligonucleotides. (A) A diagrammatic representation of the position of probing oligonucleotides used (probes 4, 5, 1, and 6) on the four different target DNA molecules. (B) Autoradiographic (left) and ethidium bromide staining (right) patterns after direct marking using probe 4. (C) Autoradiographic (left) and ethidium bromide staining (right) patterns after direct marking using probe 5. (D) Autoradiographic (left) and ethidium bromide staining (right) patterns after direct probing using probe 1. (E) Autoradiographic (left) and ethidium bromide staining (right) patterns after direct marking using probe 6. Throughout experiments, lane 1, 300-bp DNA fragment; lane 2, 500-bp DNA fragment; lane 3, 900-bp DNA fragment; and lane 4, 1200-bp DNA fragment. Nucleotide numbers (nt. numbers) are those registered in GenBank (accession no. X65328). For details, see Methods.
