Figure 4.
Deletion analysis of the minimally sufficient regulatory region. (a) The CHAOS algorithm returns four major regions of local conservation between C. savignyi and C. intestinalis. Note that this level of conservation stands out over neutrally evolving regions. (b) Plot of sequence identity between the C. savignyi and C. intestinalis TnI enhancer regions. The yellow shaded boxes represent the four CHAOS matches. (c) Horizontal lines denote sequence present in the constructs, activity is noted as in Figure 3. CHAOS matches CH1, CH2, and either of CH3/CH4 are necessary for expression of lacZ reporter in larval tail muscle. Concatenation of the four CHAOS matches is sufficient for weak lacZ expression.
