Physical map of comparative sequence analysis of the Sprr locus. (A) Representation of genes and families of genes found in the epidermal differentiation complex (EDC) on human chromosome 1q21. Sizes are in megabases from centromere. Gene order of Sprr locus are relative to flanking genes Loricrin and Involucrin. (B) Representation of genes and families of genes found in the EDC on mouse chromosome 3. Sizes are in megabases from centromere. (C) Percent identity plots (PIPs) comparing the mouse and human sequences of the Sprr locus. Analysis of the PipMaker program with the “all matches” option and the mouse sequence as the reference. The mouse genomic sequences is shown on the x-axis. Sizes are in kilobases relative to Loricrin. Percent sequence identity (50%–100%) is shown on the y-axis. In addition to the Sprr genes (introns, orange boxes; exons, blue boxes), predicted transcripts determined by BLAST are also shown (introns, yellow boxes; exons, blue boxes). The repeats of mouse sequence are depicted as follows: black pointed boxes, LINE2s; light gray pointed boxes, LINE1s; dark gray pointed boxes, LTRs; black triangles, MIRs; light gray triangles, SINEs other than MIRs; dark gray triangles, other repeats; white boxes, simple repeats. CpG islands are represented by short boxes. DNaseI HS results are also depicted in the figure: probes (P) are represented by colored boxes. Restriction enzymes used for each probe are indicated in the same color to the left and to the right of each probe below the plot (Ap, ApaI; HII, HpaII; HI, HpaI; Ps, PstI; B, BamHI; Ah, AhdI; Af, AflII; Pf, PflFI; Ss, SspI; X, XmaI; S, StuI; E, EcoRV). The DNaseI Hs are represented by arrows above the plot. To improve clarity of the figure, probes that did not reveal an HS are not displayed. (D) Dot matrix comparisons of human and mouse sequence in the Sprr region. Conservation in the dot blot is represented by black dots. The x-axis has a graphical representation of the mouse sequence in which exons are represented by coloration as in Figure 1C.
