Table 1.
Deletion mutants for enhanced hydrogen production in E. coli
|
|
|
|
|
|
Secretion per hour |
||
|---|---|---|---|---|---|---|---|
| (mmol/gDW)
|
|||||||
| No. of knockouts
|
ID
|
Reaction
|
Enzyme
|
Growth rate (L/h)
|
CO2 |
H2 |
|
| 2 | A | 1. 2PG ↔ H2O + PEP | Enolase | 0.227 | 32.7 | 22.8 | |
| 2. G6P + NADP ↔ 6PGL + H+ + NADPH | Glucose-6-phosphate | ||||||
| 3 | B | 1. ADP + 4 H+ + PI ↔ ATP + 3 H+ + H2O | ATP synthase | 0.174 | 40.9 | 29.5 | |
| 2. AC + ATP ↔ ACTP + ADP | Acetate kinase | ||||||
|
|
|
3. AKG + CoA + NAD → CO2 + NADH + SUCCoA
|
2-Oxogluterate dehydrogenase
|
|
|
|
|
-
A basis glucose uptake rate of 10 mmol/gDW per hour is assumed.
