Table 1.
Analysis of gene amplifications that are detectable (factor of 2 amplification or more) following RCA-RCA and array-CGH: Comparison to unamplified DNA
|
CGH |
log2 gene amplifications: Unamplified DNA |
log2 gene amplifications: RCA-RCA DNA |
Percent gene amplifications detected via CGH after RCA-RCA |
|---|---|---|---|
| BT-474 DNA vs. Reference DNA | >1.5 (189) | >1 (144) | 76 |
| >2 (117) | >1 (98) | 84 | |
| BT-474 DNA vs. Reference DNA: | >1.5 (85) | >1 (74) | 87 |
| Chromosome 17 | >2 (56) | >1 (52) | 93 |
| BT-474 DNA vs. Reference DNA: | >1.5 (60) | >1 (54) | 90 |
| Chromosome 20 | >2 (45) | >1 (41) | 91 |
| Paraffin #19 DNA vs. Reference DNA: | >1.5 (4) | >1 (2) | 50 |
| Chromosome 4 | >2 (2) | >1 (2) | 100 |
| Paraffin #56 DNA vs. Reference DNA: | >1.5 (3) | >1 (3) | 100 |
| Chromosome 12
|
>2 (3)
|
>1 (3)
|
100
|
-
Amplications are quoted as the log2 value. For example, for BT-474 DNA versus Reference, >1.5 (189) means that there were 189 data points with an amplification of more than 2.6-fold relative to reference. In the corresponding CGH from RCA-RCA-amplified DNA there were 144 data points with an amplification of at least two-fold.
