Figure 4.
QRT-PCR (Taqman assay)-based comparison of gene copy number ratios before and after RCA–RCA amplification for DNA extracted from BT474, paraffin #19, and paraffin #56 and from reference DNA. The relative (ΔΔCt) threshold method was used to compare relative gene copy number before amplification and after RCA–RCA amplification using the GAPDH housekeeping gene for normalization. The results are presented as copy number ratio relative to co-amplified reference DNA.
