(A) Agarose gel electrophoresis profiles of DNA extracted from reference DNA or from three FFPE samples. The first lane in each gel is a nucleic acid ladder. The reduction in average fragment size indicates that DNA degradation is progressively higher for FFPE samples #19, #56, and #31, respectively. (B) Fold-amplification obtained following whole genome amplification of reference DNA or FFPE tissue-extracted DNA, using RCA–RCA or MDA. QRT-PCR (Taqman assay) was applied to seven genes before and after amplification and the fold-amplification relative to unamplified DNA was determined by threshold comparison. MDA yielded practically no amplification for the three FFPE samples.
