Performance metrics
|
Metrica |
Value |
Confidence |
|---|---|---|
| Input Requirement (Total RNA) | 100 ng | n/a |
| Limit of Detectionb | ∼0.13 pM | 99% |
| Specificityc | ∼1:250,000 | 99% |
| Precisiond | ∼1.3-fold | 95% |
| Dynamic Rangee | ∼3.2 Logs | n/a |
| Array-to-Array %CVf
|
<10%
|
n/a
|
-
↵a The median value of nine spike controls is given for each performance metric. All metrics listed derive from measurements made using two probe sequences per gene
-
↵b Limit of detection is determined by a negative control detection model (see Methods)
-
↵c Specificity is determined by dividing the number of molecules detected at the limit of detection by the number of molecules present in 1 μg of sample background. The average mammalian transcript length is estimated at 2000 nt
-
↵d Precision is the smallest change in concentration that can be detected with 95% confidence. Value given is the median across the dynamic range
-
↵e Dynamic range is defined by the ability to detect twofold changes in target concentration across the specified concentration range (see Methods)
-
↵f Array-to-array %CV is determined using a common population of all detectable bead-types across the 96 arrays of an array matrix
