Sample labeling reproducibility. (A) Twenty sample labeling reactions were processed using our standard conditions with 10, 20, 50, 150, or 500 ng of total mouse spleen RNA as input material (four replicates each). For each input amount, correlation values (R2) of gene signals were determined for all pairwise comparisons of all successful replicates. The median R2 values, with ranges, are plotted. (B) Representative scatter plot of the intensity values for all genes measured in one of the 50-ng samples vs. those in the 500-ng sample. Whereas initial sample input varied for each labeling reaction, final array hybridization was performed using 1 μg of each labeled sample at a final concentration of 25 ng/μL.
