Figure 2.
Arrangement of spiked samples for hybridization. Each sample was produced by adding labeled spike controls to labeled complex RNA derived from human HepG2 poly(A+) RNA. The spike controls were added at the pM concentrations indicated in the figure. All nine spiked mRNAs were present at the same concentration within a given sample (e.g., 200 pM in sample a1). Samples were arranged in a staggered fashion to avoid the possibility of row/column positional bias. Hybridization was performed using 1 μg of each sample at a final concentration of 25 ng/μL.
