Table 1.
Results for RT-PCR tests of two-intron targets from TWINSCAN predictions
|
|
Targeted |
Experimentally determined |
Prediction correct |
Correct over determined |
|---|---|---|---|---|
| Intron | 216 | 147 | 128 | 0.87 |
| Exon | 108 | 63 | 49 | 0.78 |
| Splice Site
|
432
|
294
|
271
|
0.92
|
-
“Targeted” is the number of predicted introns, exons, or splice sites between the PCR primers. “Experimentally determined” is the number of each that we could determine unambiguously by aligning high-quality experimental sequence back to the genome. “Prediction correct” is the number of experimentally determined introns, exons, or splice sites that match the prediction exactly. “Correct over determined” is the number of predicted features that were verified as a fraction of the number that could be determined experimentally.
