Figure 4.
A curated annotation (blue), blocks of ungapped alignment from the genome sequence of Serotype A Strain H99 (black), the TWINSCAN prediction (red), and the PCR primers and experimental sequence aligned back to the genome (green). TWINSCAN's prediction of the missing exon is influenced by both the long ungapped alignment from H99 and the unusually (though not impossibly) long intron in the curated gene structure.
