Table 1.
Primers used in VSG expression site analysis.
|
Name |
Sense primer |
Antisense primer |
Acc. no. |
Coordinates |
|
|---|---|---|---|---|---|
| TAR T1/T2 | TACGCGTCGACTGAGGTAAGGAATATCGACG | TACGCGTCGACATAAATCGAATACGG | AL671259 | 71184-71742 | |
| ESAG7a | TTGGTTTGTGCTGTTGGCTC | CACTTCATGGAGTTGCAAGC | AL671259 | 74776-75346 | |
| ESP S1/S2 | ATATCCCTATTACCACACCA | CAATACCCACCCATACACAG | AL671259 | 71589-74112 | |
| ESP S3/S4 | CAGGGGAGGTTATTACAGAA | CCGTTTGGGCCATTTACACA | AL671259 | 71619-73768 | |
| ESAG6amp | CAGTATTGAGGAATGAGTTTA | GTTCACTCACTCTCTTTGACA | AL671259 | 77401-78159 | |
| ESAG6seq | CCAGCAGGAGTTGGAGGAAAT | CTCTGTAGCATCACCGTATTC | AL671259 | 77426-77918 | |
| ESAG1-1 | TTGAAATTGTGGAATTGGTTG | GACATCCTCCCACACTGTCC | AL671259 | 118926-119416 | |
| ESAG1-2 | ATGCGGAAGTCAAAAGGTTG | CCTCGGTCTTGAGTTTAGTGG | AL671259 | 119449-119882 | |
| ESAG1-3 | TCTCCGTTGTCTTTCGGATG | GAAATTGCTATGGACTTCGTTG | AL671259 | 119053-119845 | |
| ESAG2-1 | GCGAGTTACGAATGGTAGGC | GTCTCCGTAAGAACCGCAAC | AL671259 | 116570-117071 | |
| ESAG2-2 | CCGCTGGGGACTATAACCTAAC | ATGACTGTCAGCCTCCTTGG | AL671259 | 117205-117689 | |
| ESAG2-3 | CCTTTAAGAATCGTGAGAAGGA | TAATCACATCCCATGCCTCA | AL671259 | 116707-117390 | |
| ESAG3-1 | TGAAATTTGGTACCTCGAGAGC | CCAACTGAGGGGCATAACAC | AL671259 | 99689-100189 | |
| ESAG3-2 | ATCACAAGGAGCATGTGCAG | CGAAATTCCCACTTCTCTCC | AL671259 | 100208-100730 | |
| ESAG3-3 | GCAGAATACTGCGGAAAATGA | TCACCCCACTACTGGGAATAG | AL671259 | 100077-100555 | |
| ESAG4-1 | TCCACTTTTATCCGACAATGG | GGATTGGTGCCTGTGAAAAG | AL671259 | 88548-89025 | |
| ESAG4-2 | TTGAGGGAGAGTTGATGGTG | GTTCGCTGCCTCAGCTATG | AL671259 | 89167-89663 | |
| ESAG4-3 | GGCTTCAACGCCTCACTG | GTCCTTCGCAAGCGGATTG | AL671259 | 88308-89057 | |
| ESAG5-1 | TGTTGTATCATCGGCTCTGG | GGCATCTTTGATTTCCCTTC | AL671259 | 81975-82478 | |
| ESAG5-2 | GATAAGGATGTGCTTCCCAAG | CAGGTCCACGGCAACTTC | AL671259 | 82513-83001 | |
| ESAG5-3 | ATTCAAGAGGCGGCTGTTAC | AGCACGACCAGTTCAAGTCTCAT | AL671259 | 82056-82670 | |
| ESAG6-1 | ACCGCCGCTAATAAAGTGTG | GGAGTTGCAAGCTTTTCTGC | AL671259 | 77205-77691 | |
| ESAG6-2 | GCAGAAAAGCTTGCAACTCC | AACCTCTTTCTGCCCTATGC | AL671259 | 77672-78077 | |
| ESAG6-3 | CATTCCAGCAGGAGTTGGAGG | TTGTTCACTCACTCTCTTTGACAG | AL671259 | 77422-78161 | |
| ESAG7-3 | CATTCCAGCAGGAGTTGGAGG | TTAGGTTCCCCGCCACAC | AL671259 | 75069-75527 | |
| ESAG8-1 | CAGATGGCGAGGAAACTAGG | CACCTTCAGCTTCCCATCAG | AL671259 | 97088-97612 | |
| ESAG8-2 | ACTGCTATTGGGGGTGTGAG | CAATCCACTCAGGCTTGTTATTC | AL671259 | 97649-97143 | |
| ESAG8-3 | GTATCGGAGGGAGACGGAAG | CTGCTTCCCATCAGGATGAA | AL671259 | 96981-97604 | |
| ESAG10-2 | CACAAGCTCACGAAGCTGAG | ACCAAACCCAACTGACAAGC | AL670322 | 25506-26093 | |
| ESAG10-3 | TATGATTGGGGCTGTTGGTGGTGT | TAGACGCGCCGCTGGTATCA | AL670322 | 26752-27249 | |
| ESAG11-1 | GGGGCTGAAACATTATGTAACC | GTCGTCATCGACACTTCTCTTG | AJ239059 | 141-1055 | |
| ESAG11-2 | AATAGTGCAGGGTTTGGAGAA | CCATTTGCAACCTCTTCCGTA | AJ239059 | 287-766 | |
| VSG 221 | GCATGCCTTCCAATCAGGAGGC | GCTGTATCGGCGACAACTGCAG | X56762 | 19-558 | |
| VSG 121 | CAATAGCAGCCGATCTAGCC | TGATGGTCGCTAAGTTGTCG | X56764 | 317-808 | |
| VSG V02 | CAGCGGCTGTACATACTAAC | TAGCGTGCACATTTTCGCG | G. Rudenko, and P. Borst, unpubl. | ||
| VSG 1.8 | AAGGAGACACAATCGCCAAG | CCGTCTTTGACGCAATAGTTC | N. Aitcheson, P. Borst, and G. Rudenko, unpubl. | ||
| VSG 800 | ACTACAGACCGCCGACAGTATC | GGGCTACTAGCAGATTGCATTT | S. Hajduk, pers. comm. | ||
| VSG bR-2 | GTGAAGTCCACAAAAGACAGTGG | CGCGAGCTTGTATTCTTTTAGC | G. Cross, pers. comm. | ||
| VSG T3 | GGCACAACTTTGCTACACAATG | GGGGTCATTTATTGTCTCAAGC | G. Cross, pers. comm. | ||
| VSG 224 | CGATGACGTCAATCCAGATG | CCGTTGGTGTCGTGTCTTC | G. Cross, pers. comm. | ||
| VSG JS1
|
CCATAAAGCACGAAGCCTGGAAG
|
TAGTCCGCATCTGCCTAGTCCTG
|
N. Aitcheson and G. Rudenko, unpubl.
|
||
