Figure 7
Histidine kinase assays. Coomassie Blue staining of the recombinant proteins refolded from inclusion bodies (A). Autoradiogram of PilS after undergoing autophosphorylation and SDS-PAGE (B). Phosphorylation of a subset of response regulators by PilS (C) expressed as cpm incorporated by the substrates (corrected by the amount of substrate in each reaction).
