Figure 7
In vitro phosphorylation of arrayed proteins. Purified proteins were arrayed in quadruplicate on glass slides, and incubated with different protein kinases in the presence of [γ-33P]ATP. Rb is the retinoblastoma protein (Lee et al. 1987), which served as positive control. GFP-GST is purified fusion protein of GFP with a GST-tag, which should not be phosphorylated by the kinases. The protein from cDNA DKFZp434P097 was expressed as a fusion protein with the C terminus of GST. (A) The array was incubated with CDK2/cyclin E kinase. (B) The array was incubated with p42 MAPK kinase.
