(A) Recombinant protein expression in E. coli. The 282 Entry constructs were transferred into a His6 N-terminal fusion vector (pDEST17). Representative samples from the 282 small, medium, and long ORFs are shown. (Lanes 1-12) Plate 11006, Row F01-12, small ORFs (1) BC007838; (2) BC007355; (3) BC027953; (4) BC027899; (5) BC021719; (6) BC038108; (7) BC002826; (8) BC030277; (9) BC005991; (10) BC036723; (11) BC025403; (12) BC025760; (lanes 13-24) Plate 11023, Row A1-12, medium ORFS (13) BC001061; (14) BC000453; (15) BC000017; (16) BC001167; (17) BC001150; (18) BC000480; (19) BC001221; (20) BC001142; (21) BC002472; (22) BC000723; (23) BC000770; (24) BC001665; (lanes 25-36) Plate 11025, Row C1-12, large ORFS (25) BC037313; (26) BC035818; (27) BC007670; (28) BC007897; (29) BC012177; (30) BC037491; (31) BC005033; (32) BC032597; (33) BC036216; (34) BC001571; (35) BC012064; (36) BC034237. The positions of molecular weight markers (31-98 kDa) are indicated. All visible proteins migrate at the expected size. (B) Recombinant protein expression in mammalian cells. The 282 Entry constructs were transferred into a GFP C-terminal fusion vector (pcDNA-DEST47). Expression of GFP fusion proteins were assessed in transiently transfected 293T cells. Shown are 10 representative images of GFP fusion protein expression, with GFP images (left) showing the distribution of the fusion proteins, DAPI images (middle) indicating nuclei, and the GFP/DAPI merged images (right). MGC clone numbers are indicated to the left of each panel.
