Table 1.
Summary of BP Reactions
|
BP reaction (10 μL) |
PCR product (ng) |
PCR size (bp) |
PCR product (fmol) |
KanR (cfu)a |
Correct clonesb |
|---|---|---|---|---|---|
| B4-CUP1 promoter-B1.1R × pDONR-P4-P1.1R | 10 | 332 | 45 | 86,420 | 5/5 |
| B4-STE2 promoter-B1.1R × pDONR-P4-P1.1R | 20 | 535 | 56 | 83,520 | 5/5 |
| B4-TEF2 promoter-B1.1 × pDONR-P4-P1.1R | 15 | 653 | 34 | 30,740 | 5/5 |
| B4-GPD1 promoter-B1.1R × pDONR-P4-P1.1R | 20 | 756 | 40 | 35,380 | 4/5 |
| B4-ADH1 promoter-B1.1R × pDONR-P4-P1.1R | 50 | 1535 | 49 | 10,584 | 5/5 |
| No Clonase pDONR-P4-P1.1R
|
|
|
|
116
|
|
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↵a Colony forming units per milliliter transformation obtained from transformation of 1 μL BP reaction into 50 μL competent Mach 1 cells (Invitrogen) at 1 × 108 cfu/μg pUC.
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↵b Number of correct clones vs. total number of clones analyzed based on restriction enzyme analysis of miniprep DNA. (KanR) Kanamycin resistant.
