Figure 7
Functional analysis of STE2 receptor constructs (A) Ste2 transmembrane topology (Parrish et al. 2002) and att site locations. (B) Response to α-factor treatment of yeast strains containing STE2 receptor constructs cotransformed with the FUS1-yeGFP reporter. The STE2 receptor constructs were expressed using the STE2 promoter. Relative GFP fluorescence was measured over a 16-h period. Yeast strains were assayed in the presence of 500 nM (triangles), 50 nM (squares), or absence (circles) of α-factor as described in the Methods. Symbols are as follows: wild-type Ste2 receptor (red), Ste2 receptor containing att3 (blue), and Ste2 receptor containing both attB3 and attB4 (green).
