Concerted Assembly and Cloning of Multiple DNA Segments Using In Vitro Site-Specific Recombination: Functional Analysis of Multi-Segment Expression Clones

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Figure 5
Figure 5

Overview of three-segment cloning. (A) Linkage of a 5′ Entry Clone, a standard (L1-L2) Entry Clone, and a 3′ Entry Clone and a Destination Vector (R4-R3). (B) Alternate three-segment cloning strategy using a standard Gateway Destination Vector (R1-R2).

This Article

  1. Genome Res. 14: 2111-2120

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