Figure 4
Functional analysis of transcriptional fusions. (A) Promoter-eGFP fusions and (B) promoter-STE2 receptor fusions in yeast cells co-transformed with the FUS1-yeGFP reporter. Relative GFP fluorescence was measured over a 16-h period. Yeast strains were assayed in the presence (filled symbols) or absence (open symbols) of 500 nM α-factor as described in the Methods. Promoter fusions are as follows: STE2 (Red), ADH1 (yellow), GPD1 (blue), TEF2 (green), and CUP1 (black). Yeast strains containing the CUP1 promoter fusions were assayed in the presence (black circles) and absence (black squares) of 100 μM copper sulfate.
