Concerted Assembly and Cloning of Multiple DNA Segments Using In Vitro Site-Specific Recombination: Functional Analysis of Multi-Segment Expression Clones

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Figure 2
Figure 2

Overview of two-segment cloning. (A) Linkage of a 3′ element Entry Clone (R2-L3) to standard Entry Clones (L1-L2) and cloning into a Destination Vector (R1-R3). (B) Linkage of a 5′ element Entry Clone (L4-R1) to standard Entry Clone and cloning into a Destination Vector (R4-R2). (C) Alternate two-segment cloning strategy linking two Entry Clones (L1-L3 and R3-L2) and cloning into a standard Destination Vector (R1-R2).

This Article

  1. Genome Res. 14: 2111-2120

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