High-Throughput Expression of C. elegans Proteins

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Figure 4
Figure 4

SDS-polyacrylamide gels showing the 1-L scale-up expressions of 12 soluble proteins. The expression levels in the gel are typical for the C. elegans proteins expressed in E. coli. The bands for 76D4, 76F6, 56D11, and 76F10 represents a high-level expression with yield greater than 10 mg per liter of bacterial cell culture (mg/L). The bands for 37F1, 37G9, and 37C1 represent a medium level expression from 6 to 10 mg/L, whereas the bands for 37D4, 37B8, and 37D8 represent a low level expression from 3 to 6 mg/L.

This Article

  1. Genome Res. 14: 2102-2110

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