Table 2.
Overview of Y1H Screens
|
DNA bait |
Total no. of colonies screened |
Picked colonies |
Double positives |
Retest by gap repair |
Relevant hits |
|---|---|---|---|---|---|
| AD-cDNA library | |||||
| Pfog-3 | 1.2 × 105 | 60 | 4 | — | 0 |
| Pmab-3 | 1.8 × 105 | 48 | 4 | — | 1 |
| Pmyo-2 | 1.5 × 105 | 67 | —a | 7 | 4 |
| Phlh-8 | 1.1 × 106 | 15 | —a | 3 | 3 |
| AD-TF mini-library | |||||
| Pfog-3 | 1.4 × 106 | 45 | 22 | 5 | 5 |
| Pmab-3 | 6.2 × 105 | 48 | 0 | — | — |
| Pmyo-2 | 1.2 × 106 | 41 | —a | 2 | 2 |
| Phlh-8
|
5.6 × 105
|
44
|
—a
|
0
|
—
|
-
↵a The β-Gal test could not be performed for these promoters because of high lacZ reporter self-activation.
