Figure 5
Examples of retest of Phlh-8 and Pfog-3 positives. (A) Phlh-8 retest. (B) Pfog-3 retest. Double integrant yeast cells were transformed with an empty vector (containing Gal4 AD) and PCR products containing sequences that encode potential interaction partners as described previously (Walhout and Vidal 2001). Transformants were grown on Sc-His,Ura,-Trp plates with 40 mM 3AT or spotted on YEPD plates containing a nitrocellulose filter for β-Gal assays. Controls: 1 = no DNA, 2 = circular (uncut) empty AD vector, 3 = linear (cut) AD vector.
