Figure 4
TF-promoter interactions detected by the Gateway-compatible Y1H system. DNAbait::reporter strains were transformed with Gateway Destination plasmids containing either full-length TF-encoding ORFs, or the part of these ORFs that is predicted to encode the DB, fused to Gal4 AD. An empty Gateway vector containing only Gal4 AD was used as a negative control (-). The resulting yeast transformants were grown on Sc-His,-Ura,-Trp media (upper row), Sc-His,-Ura,-Trp, containing 40 mM 3AT (middle row), or spotted on YEPD plates containing a nitrocellulose filter for β-Gal assays (lower row).
