Figure 2
A Gateway-compatible Y1H system. (A) “Non-Gateway” Y1H control assay. As a DNA bait, six copies of the murine consensus p53-binding site (p53BS) fused to the HIS3 reporter gene was used with Gal4 AD alone (-) or with AD-p53DB as prey. (B) As a DNA bait, six copies of the murine consensus p53-binding site (p53BS) fused by a multisite Gateway reaction to the HIS3 reporter gene was used with Gal4 AD alone (-) or with AD-p53DB. Here, the p53BS bait is flanked by AttB4 and AttB1 Gateway recombination sites. Cells were plated on Sc-His,-Ura media with 0, 20, or 40 mM 3AT as indicated.
