Figure 1
Creating DNAbait::reporter constructs by multisite LR cloning. (A) Gateway-cloned ORFs encoding the Y1H reporters His3 and β-galactosidase (β-Gal) were BP cloned into pDONR201 to generate HIS3 and lacZ Entry clones. DNA baits were BP cloned into pDONR-P4-P1R to generate DNA bait Entry clones. (B) The DNA baits were fused to HIS3 and lacZ by a multisite Gateway LR reaction using the DNA bait Entry clones, the Y1H reporter entry clones, and the Y1H Destination vectors to generate DNAbait::HIS3 and DNAbait::lacZ Destination clones.
