Table 3.
Examples of Novel Splice Variants Found
|
Target RefSeq ID |
Gene name |
PCR band size |
Contig size |
Gene structure |
|---|---|---|---|---|
| NM_000877 | IL1 R1 | ∼1800 bp | 1800 bp | Perfect match to RefSeq - E2a - 11 of Ensembl |
| ∼1200 bp | 1160 bp | Deletion of: part of E3, E4, E5, E6, and 19 bp of E7 | ||
| NM_001094 | ACCN1 | ∼1600 bp | 1625 bp | One validated discrepancy - Ensembl match: E2-E10 |
| ∼1300 bp | 1345 bp | E4-E10 | ||
| ∼1200 bp | 1192 bp | E4, E5, E7-E10 | ||
| NM_003160 | STK13 | ∼900 bp | 916 bp | Passed - perfect match - E1-E7 |
| ∼1100 bp | 1111 bp | E2, E3, E4 (+ 5 bp from intron 5-6), E5, E6, 16-7, E7 | ||
| ∼800 bp | 769 bp | E2, E3, E4, E6, E7 | ||
| ∼800 bp | 779 bp | E2, E3, E5, E6, E7 | ||
| NM_006212 | PFKFB2 | ∼1600 bp | 1583 bp | Passed - some validated discrepancies - E1-E6 |
| ∼1400 bp | 1390 bp | E1 - E11, E14, E15 | ||
| NM_015310 | EFA6R | ∼1650 bp | 1667 bp | Passed - perfect match - E1-E13 |
| ∼1200 bp | 1173 bp | E1, E6, E8 - E13 | ||
|
|
|
∼1200 bp
|
1216 bp
|
E1, E6 - E13
|
-
↵a E and I indicate exons and introns found in the clone inserts. For example, “E1-E11, E14, E15” means the presence of exons 1 to 11, with exon 14 and exon 15 (exons 12, 13, and 16 were not found).
