Table 2.

Examples of Acceptable Clones Found

Target RefSeq ID	Gene name	PCR band size	Contig size	Comments
NM_000871	HTR6	∼1400 bp	1401 bp	Matches ORF perfectly.^a
NM_000910	NPY2R	∼1200 bp	1203 bp	T627C^b I->I (silent),^c T978C I->I (silent)
NM_001971	ELA1	∼800 bp	822 bp	T541C Y->Y (silent), C680G L->V (Genomic validation^d - BLAT)
NM_003308	TSPY	∼1000 bp	978 bp	A161G V->V (silent), CCCG301 GGGC PR->RA (Genomic validation - BLAT).
				G458C H->Q validated by dbEST.
NM_004347	CASP5	∼1300 bp	1320 bp	G975C V->L validated by Ensembl dbSNP as an SNP.

↵a Based on RefSeq sequence.
↵b Base change in clone insert vs. RefSeq.
↵c Effect of sequence change on protein coding.
↵d Validation means confirming the change as known polymorphism, based on current databases.

Systematic Recovery and Analysis of Full-ORF Human cDNA Clones