Figure 5
Estimated numbers of RT-PCR-generated clones required on average to identify at least one acceptable clone of the indicated length (as a function of PCR cycle number). This is based on 1/15,000 error rate of the reverse transcriptase, and 1/50,000 error rate of the high-fidelity DNA polymerase used in the clone acquisition process. n50, n75, n90, and n99 indicate the predicted numbers of clones that need to be sequenced in order to find an acceptable clone with probabilities of 50%, 75%, 90%, and 99%, respectively, based on the above error rates.
