Immunogenicity, stage expression, and localization of P. falciparum proteins encoded by ORFs cloned into DNA vaccine plasmids. In all, 95 genes from Panel A were batch cloned into the VR1020-DV DNA vaccine vector, and used to immunize 5 CD-1 mice per gene. The murine sera were pooled and screened by IFAT against the sporozoite, asexual erythrocytic (trophozoites and schizonts), and sexual erythrocytic (gametocyte) stages of P. falciparum. The asexual erythrocytic stage was a mixture of early and late trophozoites and schizonts. Sporozoites were isolated from mosquitos' salivary glands and gametocytes were percoll-purified from in vitro cultures. Of the 95 constructs screened, the number of positive genes were shown. The titer range is the lowest and the highest end-point IFA dilution scored for any positive group. Numbers on the left indicate the accession number for ORFs.
