Figure 2
PCR screening BP and LR reactions. (A) Recombinant clones in the pDONR/Zeo master plasmid were screened by colony PCR using plasmid-specific primers (M13 forward and reverse). The figure shows amplified DNA products from 12 BP reactions representing different genes in groups of four colonies (a, b, c, d) per clone analyzed on a 1% agarose gel. (B) The screening of recombinants clones in the VR1020-DV destination vector was done by PCR on DNA from 4 single colonies (a, b, c, d) as well as bulk cultures (B) from the same transformation. Clones shown were picked at random. (M) 1-kb DNA extension ladder.
