Merged genes account for a substantial number of repredictions in previously cloned ORFs. (A) Example of two ORFs that have been repredicted and merged into one longer ORF. In ORFeome Version 1 (upper lane), two pairs of primers were generated for the two predicted ORFs. The black arrows represent a primer pair (mv_F09E8.3) that did not amplify the previously predicted ORF F09E8.3. The green arrows represent primers (mv_F09E8.4) that successfully amplified a truncated version of the merged prediction. Using a new primer pair (mv100_F09E8.3), designed on the merged prediction in WS100 (green arrows, lower lane), this longer ORF was successfully cloned in-frame. (B) We have attempted to clone 324 merged ORFs in ORFeome Version 3 and confirmed former mispredictions of 99 pairs and 28 triplets of ORFs, each merged into one longer prediction in WS100.
