The C. elegans genome annotation has evolved between WS9 and WS100. (A) For all ORFs that are missing in v1.1a, those with repredicted starts and/or stops in WS100 were identified. Between WS9 and WS100, 1052 ORFs have been repredicted at the start, 962 at the stop, and 694 at both ends. A total of 6146 ORFs had the exact same start and stop in the two Wormbase releases. We also identified 1524 newly predicted ORFs in WS100. (B) Venn diagram summarizing the classification of the 4232 repredicted and new ORFs based on the experimental data available. The blue oval represents all 4232 ORFs that we attempted to clone. The purple circle contains new predictions of which 713 are touched by ESTs and 811 are untouched. The large orange oval represents all ORFs touched by ESTs. The smaller oval in light yellow shows ORFs touched by OSTs. As no OST data are available for ORFs that we did not clone in ORFeome Version 1 or for newly predicted ORFs, only ORFs that we cloned out-of-frame earlier are touched by OSTs. A small portion (64) of the latter are not touched by any ESTs. Of all 4232 predicted ORFs that we attempted to clone, 34% (626 repredicted and 811 new ORFs) are not experimentally verified (untouched), whereas 66% are touched by ESTs, OSTs, or both.
