Table 2.
Correspondence Between Actual and Expected Lengths of 1274 CDNAs
|
cDNA sequence length |
Percentage of sequence lengths that fall within the size range expected for the library |
Percent of sequence lengths that fall below the size range expected for the library |
Percent of sequence lengths shorter by no more than 15% of the expected size range for the library |
|---|---|---|---|
| 0.5-1.0 kb | 100% (22/22) | 0 | 0 |
| 1.0-2.0 kb | 79% (53/67) | 21% (14/67) | 80% (11/14; ≥ 0.85 kb) |
| 2.0-3.0 kb | 76% (116/152) | 24% (36/152) | 83% (30/36; ≥ 1.7 kb) |
| 3.0-4.0 kb | 50% (139/279) | 50% (140/279) | 90% (126/140; ≥ 2.5 kb) |
| 4.0-5.0 kb | 70% (363/521) | 30% (158/521) | 68% (108/158; ≥ 3.4 kb) |
| 5.0-7.0
|
61% (142/233)
|
39% (91/233)
|
73% (66/91; ≥ 4.25 kb)
|
