
Distribution of clone sizes in the size-fractionated cDNA libraries to assess the efficacy of the method used for size-fractionation of cytoplasmic mRNA and size selection of double-stranded cDNA. Linearized plasmid DNA from each of 16 size-fractionated libraries representing size fractions from 0.5 to 1 kb to 5 to 7 kb were electrophoresed and compared with a 1-kb standard ladder (New England BioLabs). Note that the clone sizes include the 1.7-kb pYX-Asc I vector.











