Analysis of Human mRNAs With the Reference Genome Sequence Reveals Potential Errors, Polymorphisms, and RNA Editing

Table 2.

CDS Mismatch Support




≥3 Human mRNA/EST

≥3 Non-human mRNA/EST

No support
Genome only 12,000 (61.6%) 9686 (49.7%) 1249 (6.4%)
mRNA only 1339 (6.9%) 1863 (9.6%) 10,270 (52.7%)
Genome and mRNA 2925 (15.0%) 3822 (19.6%) 824 (4.2%)
Neither
3206 (16.5%)
4099 (21.1%)
N/A
  • Analysis of 19,470 mismatched base pairs in alignments of CDS regions of full-length mRNAs to the human genome sequence. Sequences are compared against other human and nonhuman mRNA and EST sequences that align at this position. Reported are the number of instances in which three or more other human (column 2) or nonhuman (column 3) mRNA or EST sequences agree with each sequence. The last column indicates how many have no other human or nonhuman mRNA or EST support at all. The first row shows the number of times only the genome has the corresponding level of support; the second row, the same for the mRNA. The third row shows how many times both the genome and the mRNA are supported at the level indicated; the last row, when neither have that level of support.

This Article

  1. Genome Res. 14: 2034-2040

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