Figure 4
Pilot study: comparison of Gateway BP and In-Fusion cloning methods. Plasmid DNA from 288 MGC clones was PCR-amplified using Platinum Pfx Polymerase (Invitrogen) with end sequences suitable for use in either the Gateway BP or In-Fusion cloning systems. The PCR products were then used in in vitro recombination reactions with the plasmids pDONR 201 (Gateway) or linearized pDNR-Dual (In-Fusion). The products of the recombination reactions were then used to transform E. coli. (White bars) Total MGC ORFs; (dark-gray bars) successful PCR reactions; (light-gray bars) successful transformations.
