Many Paths to Many Clones: A Comparative Look at High-Throughput Cloning Methods

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Figure 2
Figure 2

Overview of Clontech recombinational cloning systems. (A) Cloning of ORF PCR products by In-Fusion-mediated recombination. The yellow and orange rectangles represent the 15-bp sequence homology required for In-Fusion cloning at the ends of PCR product and the linearized vector. (B) Transfer of ORF coding sequences from the master clone to create an expression clone by Creator cloning using the Cre site-specific recombinase. The green circles represent loxP sites.

This Article

  1. Genome Res. 14: 2020-2028

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