Many Paths to Many Clones: A Comparative Look at High-Throughput Cloning Methods

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Figure 1
Figure 1

Overview of the Gateway site-specific recombination cloning system. (A) Cloning of ORF attB-PCR products by Gateway BP Clonase-mediated recombination. The blue circles represent λ att recombination sites. (B) Transfer of ORF coding sequences from the Entry vector to create an expression clone by Gateway LR Clonase-mediated recombination.

This Article

  1. Genome Res. 14: 2020-2028

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