Table 1.
Summary of Recombinational Cloning Strategies in Terms of Demonstrated Efficiency, Adaptability, and Compatibility (See Text), Relative to Currently Available Full-Length cDNA Resources
|
|
N- & C- term |
Efficiency |
Adaptability |
Compatibility |
|---|---|---|---|---|
| FL-cDNA | No | No | No | No |
| Gap repair | Yes | Yes | No | No |
| UPS | Yes | Yes | Yes | No |
| MAGIC | Yes | Yes | Yes | No |
| Gateway
|
Yes
|
Yes
|
Yes
|
Yes
|
-
References to alternative strategies are as follows: gap repair (Orr-Weaver et al. 1983; Ma et al. 1987; Oldenburg et al. 1997), UPS (Liu et al. 1998), MAGIC (Paddison et al. 2004).
