Table 2.
Cisplatin-Sensitive Tn7 Insertional Mutants
|
Strain |
Mutagenized gene |
Standard name |
Description of gene product |
UV |
Salt |
|---|---|---|---|---|---|
| TN7-70C2 | YBL008W | HIR1 | Involved in cell-cycle regulation of histone transcription | WT | WT |
| TN7-93F1 | YBR114W | RAD16 | Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4); member of the SWI/SNF family | Sens | WT |
| TN7-69D7 | YBR279W | PAF1 | RNA polymerase II-associated, nuclear protein that may serve as both a positive and negative regulator of a subset of genes, perhaps operating in parallel with Gal11p | Sens | WT |
| TN7-29F8 | YCR007C | N/A | Putative integral membrane protein, member of DUP240 gene family | WT | WT |
| TN7-12A9 | YDL125C | HNT1 | Hint homolog, member of the histidine triad superfamily of nucleotide-binding proteins | WT | WT |
| TN7-28H7 | YDL185W | TFP1 | Encodes a protein with three regions (ABC) that is spliced to yield the extein AC and the intein B; AC is a 69K vacuolar (H+)-ATPase and B is 50K site-specific endonuclease named VDE (PI-Scel) that is homologous to HO | WT | WT |
| TN7-56E10 | YDL192W | ARF1 | Implicated in signal transduction and intracellular protein transport to or within the Golgi apparatus | WT | WT |
| TN7-1G1 | YDR074W | TPS2 | Trehalose-6-phosphate phosphatase | WT | Sens |
| TN7-71D4 | YDR148C | KGD2 | Dihydrolipoyl transsuccinylase component of α-ketoglutarate dehydrogenase complex in mitochondria | WT | WT |
| TN7-37F4 | YDR149C | N/A | Unknown gene product | WT | Sens |
| TN7-12E10 | YDR207C | UME6 | Regulator of both repression and induction of early meiotic genes. Ume6p requires Ume4 for mitotic repression and interacts with and requires Ime1p and Rim11p for induction of meiosis-specific transcription | Sens | WT |
| TN7-17H11 | YDR225W | HTA1 | Histone H2A | WT | WT |
| TN7-73H9 | YDR443C | SSN2 | Required for stable association of Srb10p-Srb11p kinase with RNA polymerase holoenzyme; regulates YGP1 expression; component of RNA polymerase II holoenzyme and Kornberg's mediator (SRB) subcomplex | Sens | Sens |
| TN7-44F7 | YDR448W | ADA2 | Transcription factor, member of ADA and SAGA, two transcriptional adaptor/HAT (histone acetyltransferase) complexes | WT | WT |
| TN7-70F9 | YDR450W | RPS18A | Homology to rat S18 and E. coli S13 | WT | WT |
| TN7-32H8 | YDR487C | RIB3 | Riboflavin biosynthesis | WT | WT |
| TN7-30D9 | YEL018W | EAF5 | Esa1p-Associated Factor-5 | Sens | Sens |
| TN7-9H11 | YER151C | UBP3 | Possible role for UBP3 in controlling the activity or assembly of the SIR protein complex | WT | WT |
| TN7-1F5 | YGR254W | ENO1 | Enolase I | WT | WT |
| TN7-42C4 | YGR258C | RAD2 | Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein | Sens | WT |
| TN7-48D6 | YHR141C | RPL42B | Homology to rat L36a and human L36a | WT | WT |
| TN7-29G4 | YHR163W | SOL3 | Weak multicopy suppressor of los1-1 | WT | WT |
| TN7-60C8 | YIL123W | SIM1 | Putatively involved in control of DNA replication | WT | WT |
| TN7-18E8 | YIL154C | IMP2′ | Protein involved in nucleo-mitochondrial control of maltose, galactose, and raffinose utilization | Sens | WT |
| TN7-33E10 | YJL117W | PHO86 | May collaborate with Pho87p and Pho84p in phosphate uptake | Sens | WT |
| TN7-49C9 | YJR052W | RAD7 | Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) | WT | WT |
| TN7-60F6 | YKL005C | N/A | Unknown gene product | WT | WT |
| TN7-62C5 | YKL006W | RPL14A | Homology to mammalian L14 | WT | WT |
| TN7-22H5 | YKL113C | RAD27 | 5′ to 3′ exonuclease, 5′ flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family | Sens | Sens |
| TN7-30D10 | YKL129C | MYO3 | Myosin I | Sens | Sens |
| TN7-39D4 | YKR092C | SRP40 | Suppressor of mutant AC40 subunit of RNA polymerase I and III (high serine) | Sens | WT |
| TN7-9B3 | YLL043W | FPS1 | Suppressor of tps1/fdp1 and member of the MIP family of transmembrane channels; may be involved in glycerol efflux | Sens | WT |
| TN7-49F7 | YML121W | GTR1 | Involved in the function of the Pho84 phosphate transporter | Sens | Sens |
| TN7-45G8 | YMR021C | MAC1 | Metal-binding transcriptional activator | WT | WT |
| TN7-29A6 | YMR089C | YTA12 | Mitochondrial ATPase (similar to E. coli FtsH protein) that resides in the inner mitochondrial membrane | WT | WT |
| TN7-10H1 | YMR110C | N/A | Unknown gene product | WT | WT |
| TN7-66H1 | YMR190C | SGS1 | Involved in maintaining genome stability. Homologous to E. coli RecQ and human BLM and WRN protein that are defective in Bloom's syndrome and Werner's syndrome, respectively | Sens | WT |
| TN7-34F12 | YMR224C | RAD58 | Disp. for premeiotic DNA synthesis, but required for both double strand break formation and resection, synaptonemal complexes, meiotic recombination, and viable spores. Localizes to discrete sites in rad50 mutants | Sens | WT |
| TN7-69H9 | YNL021W | HDA1 | Component of histone deacetylase A | WT | WT |
| TN7-15D2 | YNL104C | LEU4 | Leucine biosynthesis | WT | WT |
| TN7-12A2 | YNL107W | YAF9 | Homolog of the human leukemogenic protein AF9; member of a yeast chromatin modifying complex | Sens | Sens |
| TN7-66D1 | YNL243W | SLA2 | Protein involved in membrane cytoskeleton assembly, required for cell polarization and endocytosis | WT | Sens |
| TN7-15A8 | YOL012C | HTZ1 | Histone-related protein that can suppress histone H4 point mutation | Sens | Sens |
| TN7-2F12 | YOL018C | TLG2 | Member of the syntaxin family of t-SNAREs | WT | Sens |
| TN7-23F2 | YOR061W | CKA2 | May have a role in regulation and/or execution of the eukaryotic cell cycle | WT | Sens |
| TN7-3A1 | YOR141C | ARP8 | Actin-related protein | WT | WT |
| TN7-2D10 | YOR346W | REV1 | Deoxycytidyl transferase involved in DNA-directed DNA polymerase activity and DNA repair mechanisms | WT | WT |
| TN7-14C3 | YPL024W | NCE4 | Negative regulator of Cts1 Expression | Sens | WT |
| TN7-54H4 | YPL060W | LPE10 | Mitochondrial protein with homology to MRS2 | WT | WT |
| TN7-61B11 | YPL138C | SPP1 | Likely involved in chromatin remodeling; member of Set1p complex | WT | WT |
| TN7-31H4
|
YPL259C
|
APM1
|
Medium subunit of the clathrin-associated protein complex
|
WT
|
Sens
|
-
For all assays, strain sensitivity indicates an optical density reading at 600 nm <50% wild type.
