The Clones With a Stong Effect in the Transport and Golgi Integrity Assays
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Relative surface expression of ts-O45-Ga |
Effect on Golgi integrity |
Featuresb |
Orthologs |
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| Accession No. and clone IDs
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N-terminusc |
C-terminus
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N-terminus
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C-terminus
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TM/soluble
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domains, motifs
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M. musculus |
D. melanogaster |
C. elegans |
S. cerevisiae |
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| Secretory pathway | ||||||||||||||||
| AL080121 |
−0.0096e ± 0.0017f |
−0.0116 ± 0.0073 |
no effect | no effect | 1 TM | AAH06694 | no | no | no | |||||||
| hfbr2_2308d | 0.551 ± 0.271 | 0.371 ± 0.152 | 63%g | |||||||||||||
| DKFZp564O0823 | ||||||||||||||||
| AL136711 |
−0.0100 ± 0.0027 |
n.d.h | no effect | n.d. | 6-7 TM | AAH04013 | AAF46618 | CAA21543 | no | |||||||
| hfkd2_3i13 | 0.398 ± 0.150 | 97% | 74% | 68% | ||||||||||||
| DKFZp566l133 | ||||||||||||||||
| Golgi complex | ||||||||||||||||
| AL713668 | n.d. |
−0.0125 ± 0.0005 |
Int.: 0.55i | no effect | 5 TM | YIP domain | NM_145550 | NM_132613 | NM_062038 | YGL161cj | ||||||
| hamy2_10p70 | 0.378 ± 0.182 | 93% | 59% | 66% | 50% | |||||||||||
| DKFZp761P7010 | ||||||||||||||||
| AL136916 |
−0.0122 ± 0.0009 |
no effect | no effect | no effect | 4 TM | C terminal | BAB23556 | AAL48096 | no | no | ||||||
| hute1_19g19 | 0.377 ± 0.214 | “KKxx” motif | 97% | 52% | ||||||||||||
| DKFZp586G1919 | ||||||||||||||||
| AL136628 | n.d. |
−0.0100 ± 0.0028 |
n.d. | Frag.: 2.12i | soluble | coiled-coil | XP_131196 | no | no | no | ||||||
| hfbr2_2c18 | 0.421 ± 0.154 | 94% | ||||||||||||||
| DKFZp564C182 | ||||||||||||||||
| BC009485 |
−0.0091 ± 0.0018 |
−0.0097 ± 0.0023 |
Frag.: 1.97 | Frag.: 5.1 | soluble | XP_131196 | no | no | no | |||||||
| IRALp962K1924 | 0.710 ± 0.341 | 0.719 ± 0.318 | 83% | |||||||||||||
| IMAGE:3623656 | ||||||||||||||||
| AL136588 |
−0.0095 ± 0.0019 |
n.d. | Int.: 0.63 | no effect | 2 TM | no | no | no | no | |||||||
| hamy2_2d11 | 0.528 ± 0.0151 | |||||||||||||||
| DKFZp761D112 | ||||||||||||||||
| Cytoplasm | ||||||||||||||||
| AL136775 |
−0.0106 ± 0.0031 |
n.d. | Frag.: 1.52 | no effect | soluble | XP_138135 | no | no | no | |||||||
| htes3_17f10 | 0.537 ± 0.250 | 41% | ||||||||||||||
| DKFZp434F1017 | ||||||||||||||||
| AL080119 |
−0.0109 ± 0.0023 |
n.d. | Int.: 0.62 | no effect | soluble | HABP4 and RBP1 domains | AAH13665 | AAF44918 | AAB66119 | no | ||||||
| hfbr2_23m24 | 0.440 ± 0.186 | 98% | 44% | 41% | ||||||||||||
| DKFZp564M2423 | ||||||||||||||||
| AL136618 |
−0.0098 ± 0.0023 |
n.d. | no effect | n.d. | soluble | 6 ANK repeats | BAC26572 | no | no | no | ||||||
| hfbr2_23n16 | 0.579 ± 0.253 | 3 MORN repeats | 54% | |||||||||||||
| DKFZp564N1623 | ||||||||||||||||
| AL050156 |
−0.0097 ± 0.0024 |
−0.0056 ± 0.0015 |
no effect | Frag.: 1.88 | soluble | 3 TPR repeats | BAC30486 | AAF53617 | AAA83170 | YOR007C | ||||||
| hute1_20n10 | 0.603 ± 0.221 | 0.747 ± 0.156 | 94% | 49% | 57% | 45% | ||||||||||
| DKFZp586N1020 | ||||||||||||||||
| Cytoskeleton | ||||||||||||||||
| AL13660418 |
−0.0089 ± 0.0022 |
n.d. | Frag.:2.4 | n.d. | soluble | coiled-coil | AAH46960 | no | no | no | ||||||
| hfbr2_22f21 | 0.642 ± 0.0340 | 72% | ||||||||||||||
| DKFZp564F2122
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↵a Upper numbers are the slope b, those below are the ratio of relative surface expression in transfected to nontransfected cells. For more details see the Results and Methods sections.
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↵b All listed clones, except AL713668, which is predicted to be involved in secretory membrane transport, are novel and do not contain predictable signal peptides.
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↵c All clones exist in two variants: CFP fused to the N-terminus and YFP fused to the C-terminus.
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↵d Clone IDs used by http://gfp-cdna.embl.de/ (upper line) and http://www.dkfz-heidelberg.de/LIFEdb (lower line) databases, respectively.
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↵e Average value derived from independent experiments. For more details see Methods.
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↵f standard error value derived from independent experiments. For more details see Methods.
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↵g % of similarity on amino acid level derived by using “BLAST 2 sequences” program.
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↵h Not determined.
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↵i Change of average intensity or number of Golgi fragments. For more details see Methods.
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↵j Name of yeast ORF as assigned by SGD Yeast Database.
