Figure 4
Examples of the Golgi integrity assay. Cells were transfected with YFP alone (A), CFP-AL136571 (putative tethering factor Got1a; Conchon et al. 1999) (B), and the uncharacterized soluble protein BC009485-YFP (C), which cause no obvious alteration (D,G), weak alteration in Golgi complex morphology (E,H), or a strong scattering of the Golgi complex (F,I). Asterisks indicate transfected cells. Arrows in (D-F) point to Golgi structures and a higher magnification of these respective areas in (G-I). Arrows in G-I correspond to the arrows in D-F, respectively. Bar in A = 20 μm and is representative for images (A-F). Bar in (G) = 8 μm and is representative for images (G-I).
