Examples of the transport assay. Cells were treated, immunostained, and imaged as described in Methods. Cells transfected with (A) YFP alone, (D) CFP-AF220052 encoding the putative ER SNARE Use1p which has been proposed to be involved in retrograde Golgi to ER transport (Dilcher et al. 2003), CFP-AL136711 encoding the putative vacuole membrane protein (VMP1) which has been shown to be expressed in the conditions of the acute pancreatitis and inducing the formation of intracellular vacuoles (Dusetti et al. 2002), and (J) CFP-AL136588, an uncharacterized transmembrane protein. (B,E,H,K) Cells expressing ts-O45-G-YFP. (C,F,I,L) Immunostainings of ts-O45-G at the PM. Asterisks indicate transfected cells, arrows point to cells with ts-O45-G accumulated in the Golgi complex. Bar = 20 μm.
