Figure 6
Maintenance of abundance relationships in double-step amplification. Different synthesized DNA sequences (Prf-1, blue; Gzma, red; Gzmb, green) were mixed in known proportions at different ratios indicated in each panel (true ratios). These mixtures were next amplified in quadruplicate by a two-step PCR of 15 preamplification cycles. Triplicates of amplification curves for each dilution are shown. CT values between different dilutions of different genes reflected initial dilution conditions; that is, six cycles for a 64-fold difference and 12 cycles for a 4096-fold difference (data not shown).
