Identification of Antibiotic Stress-Inducible Promoters: A Systematic Approach to Novel Pathway-Specific Reporter Assays for Antibacterial Drug Discovery

Table 2.

Genes That Are Most Correlated With yjaX's Expression Pattern Across All 116 Experiments


Genea

Gene descriptionb

Pathwayc

RyjaXd

Se

Locationf

Operong
yjaX fabHA: 3-oxoacyl-acyl-carrier protein synthase III A FAS 1 21.2 — 70 1
yjaY fabF: 3-oxoacyl-acyl-carrier protein synthase II FAS 0.8481 21.2 1
yhfB fabHB: 3-oxoacyl-acyl-carrier protein synthase III B FAS 0.7798 17.2 — 51 2*
gltT H+/Na+-glutamate symport protein 0.7405 3
fabD Malonyl CoA-acyl carrier protein transacylase FAS 0.7253 16.7 4
accB Acetyl-CoA carboxylase (biotin carboxyl carrier subunit) FAS 0.7147 5
yhdO plsC: 1-acylglycerol-3-phosphate O-acyltransferase PLS 0.7007 15.5 — 72 6*
ylpC Unknown; similar to unknown proteins 0.6731 16.7 — 34 4
fabG β-ketoacyl-acyl carrier protein reductase FAS 0.6624 16.7 4
yxlF Similar to ABC transporter (ATP-binding protein) 0.6601 7*
panD Aspartate 1-decarboxylase PAN 0.6412 8
yjbW fabl: enoyl-acyl-carrier protein reductase FAS 0.6360 17.9 — 17 9*
panB Ketopantoate hydroxymethyltransferase PAN 0.6356 8
ylmB Similar to acetylornithine deacetylase 0.6318 10
plsX Involved in fatty acid/phospholipid synthesis PLS 0.6279 16.7 4
yraK Predicted hydrolase/acyl-transferase 0.6277
azlD Branched-chain amino acid transport 0.6271
ykoK Similar to Mg2+ transporter 0.6224
yobL Unknown; similar to unknown proteins from B. subtilis 0.6214
yrhI
Unknown; similar to transcriptional regulator (TetR/AcrR family)

0.6188



  • a The commonly used gene name

  • b A description of the gene product's function

  • c The genes already known to be involved in type II FAS and connected pathways (PAN, pantothenate biosynthesis; PLS, phospholipid biosynthesis)

  • d The correlation coefficient RyjaX

  • e The similarity score S for possible transcription-factor-binding sites in upstream sequences of the coregulated transcriptional units (averages of the scores S arising from matches with the palindromic motif on both DNA strands, see Methods), bold numbers indicating the operon head genes

  • f The genomic location of the those motifs, counted in base pairs upstream from the translation-start codon of the operon head gene

  • g The references to corresponding transcription units, with same numbers indicating the same operons (see text). A superscript star indicates isolated genes, without neighboring genes being coexpressed

This Article

  1. Genome Res. 14: 90-98

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