Table 1.
Reference Compendium Compounds and Their Underlying Mechanisms of Action (MOA)
|
Compound |
MOA |
MIC (μg/mL) |
Applied concentrations (μg/mL) (L; H) |
Treatment times (min) (1; 2) |
No. of replicates (condition L1/L2/H1/H2) |
|---|---|---|---|---|---|
| Novobiocin | DNA synthesis | 2.000 | 0.40; 4.00 | 10; 40 | 3/3/3/3 |
| Trimethoprim | DNA synthesis | 0.500 | 0.05; 0.50 | 10; 40 | 3/2/3/3 |
| Actinonin | Translation | 16.000 | 3.20; 32.00 | 10; 40 | 3/3/3/3 |
| Azithromycin | Translation | 1.000 | 0.20; 2.00 | 10; 40 | 3/3/3/3 |
| Vancomycin | Cell wall synthesis | 0.250 | 0.05; 0.50 | 10; 40 | 3/3/3/3 |
| Methicillin | Cell wall synthesis | 0.125 | 0.13; 0.25 | 10; 40 | 3/3/3/3 |
| Triclosan | Fatty acid synthesis | 1.000 | 0.10; 1.00 | 10; 40 | 3/0/3/2 |
| Cerulenin | Fatty acid synthesis | 16.000 | 3.20; 32.00 | 10; 40 | 2/11/0/3 |
| Polymyxin B | Unspecific | 16.000 | 1.60; 16.00 | 10; 40 | 3/2/2/3 |
| Monensin
|
Unspecific
|
4.300
|
0.43; 4.30
|
10; 40
|
2/3/3/3
|
-
Overall, 10 different compounds were used in this study, eight antibiotics belonging to four major MOA classes and two “unspecific” compounds acting on the cell membrane (see text). The last column provides the number of replicate experiments for the combinations of high versus low dosage together with short versus long time exposure (low-early/low-late/high-early/high-late; see Methods).
