Table 3.
Performance Features of Initial and Optimized Set Represented by a Combined Score for Cy3 and Cy5 Channels
|
|
|
Initial set |
Removed clones |
Optimized set |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Quality parameter |
Number filtereda |
Mean |
Std. dev. |
Mean |
Std. dev. |
Mean |
Std. dev. |
|||
| Signal variance | 148 | 0.5 | 0.1 | 0.3 | 0.1 | 0.5 | 0.1 | |||
| Mean-median correlation | 73 | 0.8 | 0.2 | 0.3 | 0.8 | 0.8 | 0.1 | |||
| Percent pixels >2 SD above background | 203 | 88.8 | 10.2 | 58.2 | 13.8 | 90.1 | 7.1 | |||
| Ratio std. dev. >0.2 | 336 | 1.1 | 0.5 | 1.5 | 1.4 | 1 | 0.1 | |||
| Total
|
373
|
|
|
|
|
|
|
|||
-
↵a Many clones were removed based on multiple criteria
